ARCHIVES
VOL. 8, ISSUE 1 (2026)
Development and characterization of Grateloupia lithophila silver nanoparticles and their in-vitro anti-gastric cancer assessment compared to ethanolic extract of Grateloupia lithophila using AGS cell line
Authors
Vinothini M, Dr. Sethuramani A
Abstract
Marine red algae represent an invaluable source of structurally diverse
secondary metabolites with significant pharmacological potential. The present
study investigates the anti-gastric cancer efficacy of Grateloupia
lithophila against human gastric adenocarcinoma (AGS) cell lines through
the MTT assay, employing both ethanolic extract (EEGL) and a green-synthesized
silver nanoparticle formulation derived from the aqueous extract (AgNPs-AEGL).
The silver nanoparticles were prepared using an eco-friendly reduction process
and characterized by UV-Visible spectroscopy, FT-IR and scanning electron
microscopy (SEM), confirming nanoscale formation and surface stability.
Comparative cytotoxic assessment revealed a concentration-dependent reduction
in AGS cell viability, with IC₅₀ values of 76.43 μg/mL for EEGL, 34.23 μg/mL
for AgNPs-AEGL, and 25.93 μg/mL for the standard chemotherapeutic agent
5-fluorouracil (5-FU). The enhanced cytotoxic potential of AgNPs-AEGL is v
cellular shrinkage, membrane blebbing, and nuclear condensation corroborated
apoptotic cell death. These findings highlight the synergistic interplay
between the phytochemical matrix of G. lithophila and nanostructured
silver, emphasizing its promise as a potent marine-derived nanotherapeutic for
gastric carcinoma management. The study establishes a strong pharmacological
rationale for further mechanistic and in vivo evaluation of G.
lithophila-based formulations in cancer therapeutics.
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Pages:35-44
How to cite this article:
Vinothini M, Dr. Sethuramani A "Development and characterization of Grateloupia lithophila silver nanoparticles and their in-<i>vitro</i> anti-gastric cancer assessment compared to ethanolic extract of Grateloupia lithophila using AGS cell line". International Journal of Medical Science and Research, Vol 8, Issue 1, 2026, Pages 35-44
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