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VOL. 7, ISSUE 2 (2025)
In-vivo cardioprotective activity and in vitro antioxidant activity of leaves extract of Pergularia Deamia
Authors
Prakash P N, Daniel Raj N, Gayathri S, Maduvarshan N B, Tamilarasi G
Abstract
The present study investigates the in vivo cardioprotective and in
vitro antioxidant activities of the ethanolic extract of Pergularia
daemia leaves (EEPDL). Cardiac arrhythmia was experimentally induced in Daphnia
magna using 50 mM lactose to create oxidative stress and calcium imbalance.
Treatment with varying concentrations of EEPDL (0.5–2.0 µl/ml) significantly
restored normal heart rhythm in a dose-dependent manner, with the highest dose
(2 µl/ml) showing a heart rate comparable to metoprolol (25 µg/ml), indicating
strong cardioprotective efficacy (p < 0.001). The in vitro
DPPH radical scavenging assay demonstrated potent antioxidant activity with
77.53% inhibition at 100 μg/ml and an IC₅₀ of 0.98 μg/ml, suggesting the
presence of phenolic and flavonoid compounds responsible for free radical
neutralization. These findings highlight that Pergularia daemia
possesses potent antioxidant and cardioprotective properties, supporting its
traditional use in treating oxidative stress–related cardiac disorders.
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Pages:117-121
How to cite this article:
Prakash P N, Daniel Raj N, Gayathri S, Maduvarshan N B, Tamilarasi G "<i>In-vivo</i> cardioprotective activity and <i>in vitro </i>antioxidant activity of leaves extract of <i>Pergularia Deamia</i>". International Journal of Medical Science and Research, Vol 7, Issue 2, 2025, Pages 117-121
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