Pregnancy is characterized by so many anatomical and physiological adaptations, of which the kidney is implicated. However, no much attention has been given to this aspect, especially in Northern Nigeria. The study compared the serum creatinine levels of pregnant women across the trimester and its correlation with glomerular filtration rate. This study is a comparative cross-sectional study. Sixty-one (61) pregnant women were enrolled. The comparisons of serum creatinine among trimester groups were performed. Serum creatinine was measured by Jaffe’s end-point and Jaffe’s Kinetic method respectively. A significant negative correlation was recorded between Glomerular filtration rate and serum creatinine. A significant decrease was noticed in creatinine levels using the Jaffe’s Kinetic when compared with Jaffe’s endpoint method. This study finds that the decrease in serum creatinine levels in pregnant women is normal physiological changes. However, an abnormal increase is a pointer to pregnancy related acute kidney Injury.