Green synthesis method for nanoparticle production is gaining more important because of adverse effect of use of toxic chemicals for nanoparticles synthesis. An alarming increase in fungal strains resistant to existing antifungal agents demands a renewed effort to seek agents effective against pathogenic fungus resistant to antifungal agents. The aim of this study was to assess the antifungal activity of different concentrations of AgNO3 solution, AgNPs synthesized by the wet chemical method and green method and aqueous extract of medicinal plant Nigella sativa seeds using disk diffusion assay on selected laboratory isolates of fungus. The aqueous extract of Nigella sativa were screened for their antifungal activity against laboratory isolates and the aqueous extract of Nigella sativa seeds showed pronounced dose dependant antifungal activity on laboratory isolates of Aspergillus niger and Fusarium Oxysporum.