Background: Diabetes is a chronic endocrine disorder affecting the body‘s metabolism and resulting in structural changes affecting the organs of the vascular system. Serious complications resulting from diabetes include coronary heart disease, stroke, retinopathy, renal failure, peripheral artery disease, and neuropathy. Diabetic foot ulcer is one of the complications associated with diabetes mellitus characterized by the triad of neuropathy, infection and ischaemia. Aim of the study: To detect the most common type of bacteria accompanied with diabetic foot ulcer and to study the antibiotic susceptibility on these bacteria in vitro. Patients and method: A cross sectional descriptive study was conducted at Al-Kindy Teaching hospital in the period from the first of May, 2017 to the end of April 2018.Convenient sample of 100 diabetic foot patients their ages ranges from (<40 _ >70) years old, who were willing to participate in this study and were available at the time of data collection both sex were included. Results: Mean age of the respondents were (54.9±10.2) years, (58%) with primary level of education, (83%) of them were unemployed. Most (97%) diabetic foot patients were type 2 DM, and (76%) of them had high HbA1c level. The main culture sensitivity results were; Proteous (38.0%), Pseudomonaus aerogenousa (19.0%), Staphylococcus aureus (11.0%), the Antibiotic sensitivity test was done for (87) diabetic foot patient and results revealed that Meroponim sensitivity was (97.7%) of diabetic patients, Azethromycin sensitivity was (67.8%) of patients, Gentamycin sensitivity was (65.5%) of patients, Amikacin sensitivity was (90.8%) of patients, Ceftriaxone sensitivity was (47.1%) of patients and Vancomycine sensitivity in (48.3%) of patients. Conclusion: Gram negative bacteria were more predominant and the Proteous is the main microorganism then, Pseudomonaus aerogenousa, Staphy aureus, Klebsila, E. coli., High levels of resistance to ceftriaxone and vancomycin were found.