Introduction: Cerumen contains various antimicrobial factors which exhibit a wide spectrum of activity against various pathogenic bacteria and form a complex of innate biochemical protection. Antimicrobial peptides are thought to have an important role in defense against infection. Objective: to know the antimicrobial activity of ear ear wax. Methods: The literature study used in vivo, in vitro, in silico research articles, and literature review articles from national and international journals accessed from Google Scholar, Elsevier, Science Direct, and Pubmed using the keywords antimicrobial, cerumen, ear. We used 13 relevant articles in this literature review. Results: The natural defenses of cerumen involve innate immunity including a large number of antimicrobial peptides and proteins. These antimicrobial peptides and proteins include human B-Defensin (hBD), LL-37, dermcidin, lysoferrin, lactoferrin, secretory secretory leukocyte proteases (SLPI, antileukoprotease), and alpha 1-antitrypsin. These proteins and peptides exhibit broad spectrum activity against various microorganisms. Conclusion: The peptides and proteins contained in cerumen show broad spectrum activity against various microorganisms.