International Journal of Medical Science and Research
2019, Vol. 1, Issue 1
Anti-diabetic effects of methanol extract of Dennettia tripetala on diabetic mice
With recent advances in Diabetes research ranking it the eighth leading cause of death across the globe, it has become imperative to advance and harness the use of herbal medicines to better the lots of its sufferers. This study was hitherto designed to evaluate the anti-diabetic effects of methanol leaf extract of Dennettia tripetala on alloxan induced diabetic mice. A total of sixty five (65) mice rats with confirmed evidence of diabetes (following induction with alloxan monohydrate) were randomly grouped into thirteen (13) of five mice per group. Animals were then given various treatment as follows; Group 1 (normal control), group 2 (diabetic control), group 3 (received 5 mg/kg of Glibenclamide, a known anti-diabetic agent) and groups 4 -13 were given 250 and 500 mg/kg doses of Methanol Extract [ME], N-Hexane Fraction [NH], Ethyl Acetate Fraction [EAF], Butanol [BF] and Water [WF] Fractions respectively. After period of administration of test substances, Fasting blood glucose (FBG) levels were determined (before and after test periods) using a glucometer, and the lipid profile levels were assayed with the aid of spectrophotometer. Statistical comparisons of mean differences revealed that extract treated group [ME] had a statistically significantdecrease (p < 0.05) in FBG levels within 10 hours of acute treatment and 14 days short term treatment. Also, inhibitory concentration (IC50) of the methanol extract and fractions of Dennettia tripetala samples was observed to significantly increase in the following order: Ascorbic acid (3.00 ug/ml) < BF (29.13 ug/ml) < NF (30.03ug/ml) < EAF (30.05 ug/ml) < ME (32.03 ug/ml) and WF (40.16 ug/ml). Lastly, Histological examination showed a marked and mild rejuvenation of the pancreatic β cells of diabetic mice treated with methanol extract and fractions of Dennettia tripetala, suggestive that Dennetia tripetala could be anti-diabetic.